FluoCa Wide-Filed Optical System for Awake Mice. FluoCa “awake imaging” solution FC-MHC-V5 for multi-channel wide-field optical recording and optogenetic stimulation of a conscious rodent’s brain). integrates Neurotar’s proprietary head-fixation platform that eliminates the biases associated with the use of general anesthetics, alleviates the stress experienced by animals under common head- fixation conditions, and enriches the environment by exposing the animals to a variety of tactile, olfactory and visual stimuli. A magnetic tracking device built into the AirLift Large allows real-time monitoring of the mouse’ location and velocity, with a possibility to trigger automated delivery of an optogenetic light pulse, liquid reward, air puff, electric or sensory stimulus.
Key components:
• Sturdy aluminum alloy frame for stable operation of optical components;
• AirLift Large for stress-free two-dimensional locomotion of a head-fixed behaving mouse;
• Magnetic tracking device for real-time monitoring of mouse location and trajectory, with programmable automatic triggering of reward or stimulation;
• Scientific-grade sCMOS camera (4.2 megapixel, 100 fps) for fast interleaved imaging of 4 fluorescence channels (e.g., GCaMP6,, tdTomato,Cy3 & Cy5);
• VisiView software;
• Two sets of manual controlled XYZ translationstage;
• 6 wavelength LED illumination channels;
• 10 stainless steel head plates.
FluoCa Wide-Filed Optical System for Awake Mice. FluoCa “awake imaging” solution FC-MHC-V5 for multi-channel wide-field optical recording and optogenetic stimulation of a conscious rodent’s brain). integrates Neurotar’s proprietary head-fixation platform that eliminates the biases associated with the use of general anesthetics, alleviates the stress experienced by animals under common head- fixation conditions, and enriches the environment by exposing the animals to a variety of tactile, olfactory and visual stimuli. A magnetic tracking device built into the AirLift Large allows real-time monitoring of the mouse’ location and velocity, with a possibility to trigger automated delivery of an optogenetic light pulse, liquid reward, air puff, electric or sensory stimulus.
Key components:
• Sturdy aluminum alloy frame for stable operation of optical components;
• AirLift Large for stress-free two-dimensional locomotion of a head-fixed behaving mouse;
• Magnetic tracking device for real-time monitoring of mouse location and trajectory, with programmable automatic triggering of reward or stimulation;
• Scientific-grade sCMOS camera (4.2 megapixel, 100 fps) for fast interleaved imaging of 4 fluorescence channels (e.g., GCaMP6,, tdTomato,Cy3 & Cy5);
• VisiView software;
• Two sets of manual controlled XYZ translationstage;
• 6 wavelength LED illumination channels;
• 10 stainless steel head plates.
