FluoCa Wide-Filed Optical System for Awake Mice. FluoCa “awake imaging” solution FC-MHC-V5 for multi-channel wide-field optical recording and optogenetic stimulation of a conscious rodent’s brain). integrates Neurotar’s proprietary head-fixation platform that eliminates the biases associated with the use of general anesthetics, alleviates the stress experienced by animals under common head- fixation conditions, and enriches the environment by exposing the animals to a variety of tactile, olfactory and visual stimuli. A magnetic tracking device built into the AirLift Large allows real-time monitoring of the mouse’ location and velocity, with a possibility to trigger automated delivery of an optogenetic light pulse, liquid reward, air puff, electric or sensory stimulus.

Key components: 

• Sturdy aluminum alloy frame for stable operation of optical components;

• AirLift Large for stress-free two-dimensional locomotion of a head-fixed behaving mouse; 

• Magnetic tracking device for real-time monitoring of mouse location and trajectory, with programmable automatic triggering of reward or stimulation; 

• Scientific-grade sCMOS camera (4.2 megapixel, 100 fps) for fast interleaved imaging of 4 fluorescence channels (e.g., GCaMP6,, tdTomato,Cy3 & Cy5);

• VisiView software; 

• Two sets of manual controlled XYZ translationstage; 

• 6 wavelength LED illumination channels; 

• 10 stainless steel head plates.

产品介绍：

高速三维荧光成像在生命科学研究中具有重要应用，传统的点扫描共聚焦显微镜虽然具有较好的图像分辨率，但受限于扫描方式，造成成像速度慢，光损伤强度，很难满足高通量的三维成像需求。
OSM7（FluoCa optical sectioning Microscopy system）采用旋转盘共聚焦技术，可以同时扫描多个激光焦点，然后通过面阵检测器：比如sCMOS相机来采集图像信号。这样整体成像速度得到了极大提高，对样品的光漂白、光毒性也能减少很多。从而使得转盘共聚焦显微镜成为高通量三维成像的最佳工具。

产品特点：

OSM7支持宽场荧光成像和转盘共聚焦成像一键切换；

同时拥有对角线22mm的标准光学视野；

通过C接口与主流品牌显微镜厂家的正、倒置显微镜快速安装；

光源支持LED荧光光源光导管输入或者固体激光器多模光纤输入两种选择。

产品组成：

多点扫描盘和 多通荧光滤色镜组、多色固体激光器光源/6色LED荧光光源、实时控制器、高灵敏度sCMOS相机、计算机系统和显微成像分析软件等；

产品参数：

FluoCa Wide-Filed Optical System for Awake Mice. FluoCa “awake imaging” solution FC-MHC-V5 for multi-channel wide-field optical recording and optogenetic stimulation of a conscious rodent’s brain). integrates Neurotar’s proprietary head-fixation platform that eliminates the biases associated with the use of general anesthetics, alleviates the stress experienced by animals under common head- fixation conditions, and enriches the environment by exposing the animals to a variety of tactile, olfactory and visual stimuli. A magnetic tracking device built into the AirLift Large allows real-time monitoring of the mouse’ location and velocity, with a possibility to trigger automated delivery of an optogenetic light pulse, liquid reward, air puff, electric or sensory stimulus.

Key components: 

• Sturdy aluminum alloy frame for stable operation of optical components;

• AirLift Large for stress-free two-dimensional locomotion of a head-fixed behaving mouse; 

• Magnetic tracking device for real-time monitoring of mouse location and trajectory, with programmable automatic triggering of reward or stimulation; 

• Scientific-grade sCMOS camera (4.2 megapixel, 100 fps) for fast interleaved imaging of 4 fluorescence channels (e.g., GCaMP6,, tdTomato,Cy3 & Cy5);

• VisiView software; 

• Two sets of manual controlled XYZ translationstage; 

• 6 wavelength LED illumination channels; 

• 10 stainless steel head plates.

产品介绍：

高速三维荧光成像在生命科学研究中具有重要应用，传统的点扫描共聚焦显微镜虽然具有较好的图像分辨率，但受限于扫描方式，造成成像速度慢，光损伤强度，很难满足高通量的三维成像需求。
OSM7（FluoCa optical sectioning Microscopy system）采用旋转盘共聚焦技术，可以同时扫描多个激光焦点，然后通过面阵检测器：比如sCMOS相机来采集图像信号。这样整体成像速度得到了极大提高，对样品的光漂白、光毒性也能减少很多。从而使得转盘共聚焦显微镜成为高通量三维成像的最佳工具。

产品特点：

OSM7支持宽场荧光成像和转盘共聚焦成像一键切换；

同时拥有对角线22mm的标准光学视野；

通过C接口与主流品牌显微镜厂家的正、倒置显微镜快速安装；

光源支持LED荧光光源光导管输入或者固体激光器多模光纤输入两种选择。

产品组成：

多点扫描盘和 多通荧光滤色镜组、多色固体激光器光源/6色LED荧光光源、实时控制器、高灵敏度sCMOS相机、计算机系统和显微成像分析软件等；

产品参数：