The pE-300ultra is the most controllable member of the pE-300 Series. In addition to the many features of the pE-300lite and the pE-300white, the system offers precise control over wavelength intensity and shuttering. Until now these benefits have only been accessible to users of high end, highly flexible illuminators such as the CoolLED pE-4000.
The pE-300ultra offers triggering from multiple TTL inputs which, coupled with the ability to mount inline excitation filters, provides microsecond switching of pre-filtered excitation light. This, when paired with today’s high performance multi-band filter sets, facilitates imaging traditionally done using a white light source and a filter wheel, with all the benefits of LED, and most excitingly at speeds not previously so affordable.
As part of the award-winning pE-300 Series, the NEW Enhanced pE-300ultra now delivers DOUBLE the intensity at the microscope sample plane. It allows adjustment of output in 1% steps, giving precise control. The broad spectrum covers everyday fluorophores: DAPI, CFP, Aqua, FITC, TRITC, TxRed, Cy5 and many more.
A benefit of the pE-300
ultra is that stains can be viewed either individually or in combination, without filter cube changes. This makes it ideal for use with multi-band filter sets as the screening process can be simplified when fewer filter cubes are used. Independent control of the three LED channels means that the user can control the level of excitation of each fluorescent stain independently on a multi-stained sample, potentially removing the need for single band filter sets altogether. For further information on improved multi-stain testing, go to the Downloads tab.
An important additional feature of the pE-300
ultra is that it includes CoolLED’s “
Sequence Runner” multiple channel excitation mode. Users can define the order of their fluorophore capture using their pE-300
ultra Control Pod, then the pE-300
ultra light source can accept a single TTL output from the experiment set-up’s camera to initiate the step-through of a sequence of excitation channels. This feature is independent of the individual channel TTL inputs on the light source. This offers users the facility to run through a sequence of excitation channels using a camera which has only a single TTL-out.
Power requirements
100-240V a.c. 50/60Hz, 1.4A
Power consumption
pE-300 Series
Light Source
At the wall (includes power supply unit consumption)
Standby
2W
3.3W
1 band (GYR)
20W
24.4W
2 bands (BLU & GYR)
38W
44.3W
Full white intensity (3 bands)
46W
56.4W
Dimensions
pE-300ultra Light Source: 77mm(w) x 186mm(d) x 162mm(h) - Weight 1.4kg
pE-300ultra Control Pod: 88mm(w) x 125mm(d) x 37mm(h) - Weight 0.32kg
pE-300ultra Power Supply: 167mm(w) x 67m(d) x 35mm(h) – Weight 0.62kg
pE-Universal Collimator: 44mm(w) x 86mm(d) x 44mm(h) – Weight 0.17kg
Environment & Safety
LED products are more sustainable and energy efficient than conventional illuminators. CoolLED's products have the following benefits:
Mercury-free
Energy Efficient: 80% less power
Long lifetime (25,000 operating hours)
No bulb replacements
Reduced risk of eye damage
Quiet operation
No special disposal regulations or issues
Warranty
System 36 months
The award winning pE-300 Series is a range of LED Illumination Systems for Fluorescence, Optogenetics, Electrophysiology and high speed microscopy applications. It comprises 3 systems providing a solution that covers a range of everyday fluorescence microscopy; from the simple pE-300lite through the classic pE-300white to the highly controllable pE-300ultra.
CoolLED's pE-300 Series was the 2017 winner of the "Go Beyond Award" in the “Products” category for “Excellence in sustainability in laboratory and other high technology facilities” The International Institute for Sustainable Laboratories (I2SL) runs a unique awards program honouring organisations, individuals, products,and projects that are advancing sustainable, high-performance facilities.
The table below is a quick reference guide to the features offered by the pE-300 Series systems 
A benefit of the pE-300
ultra is that stains can be viewed either individually or in combination, without filter cube changes. This makes it ideal for use with multi-band filter sets as the screening process can be simplified when fewer filter cubes are used. Independent control of the three LED channels means that the user can control the level of excitation of each fluorescent stain independently on a multi-stained sample, potentially removing the need for single band filter sets altogether. For further information on improved multi-stain testing, go to the Downloads tab.
An important additional feature of the pE-300
ultra is that it includes CoolLED’s “
Sequence Runner” multiple channel excitation mode. Users can define the order of their fluorophore capture using their pE-300
ultra Control Pod, then the pE-300
ultra light source can accept a single TTL output from the experiment set-up’s camera to initiate the step-through of a sequence of excitation channels. This feature is independent of the individual channel TTL inputs on the light source. This offers users the facility to run through a sequence of excitation channels using a camera which has only a single TTL-out.
Power requirements
100-240V a.c. 50/60Hz, 1.4A
Power consumption
pE-300 Series
Light Source
At the wall (includes power supply unit consumption)
Standby
2W
3.3W
1 band (GYR)
20W
24.4W
2 bands (BLU & GYR)
38W
44.3W
Full white intensity (3 bands)
46W
56.4W
Dimensions
pE-300ultra Light Source: 77mm(w) x 186mm(d) x 162mm(h) - Weight 1.4kg
pE-300ultra Control Pod: 88mm(w) x 125mm(d) x 37mm(h) - Weight 0.32kg
pE-300ultra Power Supply: 167mm(w) x 67m(d) x 35mm(h) – Weight 0.62kg
pE-Universal Collimator: 44mm(w) x 86mm(d) x 44mm(h) – Weight 0.17kg
Environment & Safety
LED products are more sustainable and energy efficient than conventional illuminators. CoolLED's products have the following benefits:
Mercury-free
Energy Efficient: 80% less power
Long lifetime (25,000 operating hours)
No bulb replacements
Reduced risk of eye damage
Quiet operation
No special disposal regulations or issues
Warranty
System 36 months
The award winning pE-300 Series is a range of LED Illumination Systems for Fluorescence, Optogenetics, Electrophysiology and high speed microscopy applications. It comprises 3 systems providing a solution that covers a range of everyday fluorescence microscopy; from the simple pE-300lite through the classic pE-300white to the highly controllable pE-300ultra.
CoolLED's pE-300 Series was the 2017 winner of the "Go Beyond Award" in the “Products” category for “Excellence in sustainability in laboratory and other high technology facilities” The International Institute for Sustainable Laboratories (I2SL) runs a unique awards program honouring organisations, individuals, products,and projects that are advancing sustainable, high-performance facilities.
The table below is a quick reference guide to the features offered by the pE-300 Series systems
